Pathogenicity, anastomosis groups, host range, and genetic diversity of <i>Rhizoctonia</i> species isolated from soybean, pea, and other crops in Alberta and Manitoba, Canada

Root rot is a common disease in soybean (Glycine max) and field pea (Pisum sativum), which restrain increased production Canada. Sixty-seven isolates of Rhizoctonia were recovered from various diseased plants Alberta, Canada along with three Manitoba, According to their anastomosis behavior, 23 (32.9%) the identified as group (AG) 4 (AG4), 7 (10.0%) AG2-1, 10 (14.3%) AG2-2, AG5, 3 (4.3%) AG-E AGs remaining 20 (28.6%) could not be determined. Isolates belonging AG4 produced typical symptoms stem root on seedlings more aggressive than AG5 isolates. Selected AG4, some degree able infect crops included barley, canola, corn, faba bean, flax, lupin, lentil, pea, potato, soybean, wheat. The genetic variability among these was evaluated using phylogenetic analysis based rDNA ITS sequences inter-simple sequence repeat (ISSR) markers. For analysis, neighbour-joining tree constructed PAUP program, clustered into five groups (Groups I V). However, no correlation observed between AGs, locations, aggressiveness or host origins. ISSR 54 polymorphic patterns identified, indicating high level diversity